Description of articleGenetická variabilita vybraných populací borovice lesní v České republice.
[Genetic variability of selected populations of Scots pine in the Czech Republic] 223 - 229.
|Name of article:||Genetická variabilita vybraných populací borovice lesní v České republice|
|Author:||Pavlína Máchová, Helena Cvrčková, Lucie Poláková, Olga Trčková|
The Simple Sequence Repeats (SSR) method of DNA analyses were used to determine the polymorphism of Scots pine (Pinus sylvestris L.) samples. Total genomic DNA was extracted by DNA Plant Mini Kit (Qiagen) from needles taken from three marginal populations of Scots pine located on serpentines, from one population growing at high altitude (Šumava mountains) and from two core populations of Scots pine from the Czech Republic. Samples were screened using selected twelve polymorphic nuclear microsatellite markers. Measuring the size of amplification products was carried out using the genetic analyzer Applied Biosystems 3500. The obtained data were analysed using the statistical program GenAlEx 6.5 (PEAKALL, SMOUSE 2012) and Cervus (KALINOWSKI et al. 2007). Ten selected genetic loci were verified as highly polymorphic and could be further used for comparative genetic analyses of Scots pine populations.