| Name of article: | Vývoj somatických embryí chlumního ekotypu smrku ztepilého v souvislosti se změnami endogenního obsahu spermidinu, putrescinu a sperminu |
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| Number: | 3 |
| Year: | 2009 |
| Author: | Helena Cvrčková, Jana Malá, Pavlína Máchová, Milena Cvikrová |
Somatic embryogenesis is a powerful tool for improvement of forest trees and is considered to be the in vitro plant propagation system for conifers. The process of somatic embryogenesis in Norway spruce (Picea abies /L./ Karst.) can be divided into periods characterized by the different degree of embryonic tissue differentiation. Contents of free polyamines: putrescine, spermidine and spermine were determined in different developmental stages of hurst ecotype of Norway spruce (Picea abies /L./ Karst.) somatic embryos: after 4 weeks of the growth of the embryogenic tisue on proliferation medium, after 2 and 5 weeks of the culturing on maturation medium, after 1 and 2 weeks in the course of desiccation phase. Maturation of somatic embryos was accompanied by increase of concentration of polyamines. Ivory-coloured torpedo stage embryos after 5 weeks on maturation medium with not yet well developed cotyledons contained the highest level of spermidine. Two weeks after desiccation the concentration of spermidine and putrescine decreased, whereas the content of spermine significantly increased. During the desiccation phase the radicles of embryos with well-developed cotyledons started changing its colour to the red. Mainly these embryos converted into plantlets. To improve the efficiency of somatic embryogenesis of less responsive genotypes the potential application of polyamines into the growth medium is discussed.
