| Name of article: | Vliv specifického třídění semenáčků smrku na růst a genetickou diverzitu výsadeb v extrémních horských podmínkách |
|---|---|
| Number: | 2 |
| Year: | 2012 |
| Author: | Ondřej Ivanek, Jan Leugner, Antonín Jurásek |
The growth and genetic features of Norway spruce (Picea abies /L./ Karst.) plants which showed various intensity of juvenile growth were assessed in a research plot in the Krkonoše Mts. Use of one-dimensional horizontal electrophoresis on the starch gel, G-6-PDH, GDH, SDH-A, PGM-A, MDH-B, MDH-C, IDH-B, AAT-A, AAT-B and AAT-C isozyme loci was studied. Numbers of alleles per locus, observed heterozygosity were evaluated and Shannon and Rao indices of genetic diversity were calculated. The highest genetic diversity was found for AAT-C locus, the lowest one for the PGM-A, MDH-B, AAT-A and AAT-B loci for all of the spruce variants. With the aid of Monte Carlo test significant differences were found at Norway spruce variants with different growth and growth rate for the G-6-PDH and SDH-A loci. Spruces characterized by slow growth in nursery, which would have been rejected in common nursery sorting, had the best height and diameter growth after 17 years on extreme mountain site. They showed also a very good ability to cope with extreme mountain conditions as there were proved also the good slenderness ratio and greater genetic diversity of these plants in comparison with formerly fast-growing spruces in nursery and with common plantation.
