ZLV Online

Abstract

Three different possibilities of spruce bark beetle breeding methods for substances feeding toxicity assays were tested – rearing of adults on cellulose wadding, methods of bark sandwiches for larvae and adults and method of sandwiches of bark with glass combination for larvae. Analysis of variance was used for evaluation of differences of the rearing methods, cluster analysis was used for optimal terms designation af adults rearing on cellulose wadding. Methods of adults rearing on cellulose wadding and bark sandwiches cannot be considered as qualitatively equal. Three the most appropriate terms of controls were recognized in the methods of adults rearing on cellulose wadding: always between the first and second, fifth and seventh and finally twelfth and fifteenth days since the experiment started. Methods of rearing of larvae in bark sandwiches and in sandwiches of bark with glass combination were found comparable in all aspects, but primary mortality of larvae, which is statistically higher in bark sandwiches.