Name of project:Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species
Year:2007 - 2011

Description of project

Bacillus thuringiensis var. tenebrionis delta-endotoxin gene cry3A will be completely reconstructed for high expression in spruce. Modification of domain II loop 1 amino acids are planned, too, that should increase toxicity to Scolytidae species. Modified versions of cry3A genes will be expressed in E. coli and toxicity tested in vivo. Transgenic spruce embryogennic lines expressing modified cry3A genes will be developed and characterized.