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The article deals with the content of selected micro- and macronutrients in spruce and pine bark. We also investigated the difference in chemical content between intact bark and bark damaged by hoofed game. Two localities in South Bohemia were chosen for the research – shooting area Lužánky and Červený jelen, situated close to Jindřichův Hradec, where cloven-hoofed game injures trees by bark stripping and browsing. The objective of presented contribution is to publish results of chemical bark analysis with respect to investigated nitrogen substances, lipids, nitrogen free substances, nitrates, ash matter, pulp, sugars, calcium, phosphorus, magnesium, potassium, sodium and cobalt. Trees damaged by browsing of big game labour with diverse kind of decay. The penetration of infection is often lethal – many kinds of fungi cause the wood to become discoloured or to rot. Differences in content of substances and elements between healthy and damaged trees were recorded.