Obsah/Content: ZLV 4/2023
ZLV, 68, 2023, Číslo 40 - 0.
Amurk cork tree (Phellodendron amurense Rupr.) is known for its anti-inflammatory, antipyretic, cholagogue, and antibacterial properties, and it is also a potential source of industrial cork. Shoot organogenesis and subsequent plant multiplication of Amur cork tree were established from mature seeds of trees growing on research plots in the Czech Republic. Germination percentage was increased by mechanical disruption of the seed coat, the highest number of germinated plants was achieved within 8–10 weeks of culture on MS medium containing 2 mg.l-1 BAP and 0,2 mg.l-1 NAA. Within 4 weeks of transfer to the multiplication medium containing 2 mg.l-1 BAP and 0,2 mg.l-1 NAA, chlorosis and reduced growth were observed in all cultured microcuttings. Lowering the BAP concentration to 0,4 mg.l-1 led to a resumption of growth, the newly emerging shoots and leaves were vital and the multiplication rate was around 90%. Our developed plant regeneration method may be useful in future studies on ex vitro establishment of in vitro derived plants.
Using pollen analysis, phytolith analysis and radiocarbon dating of newly discovered sedimentary archives, here we prove the native status of European larch (Larix decidua Mill.) in two sandstone rocky areas of north Bohemia (Czech Republic). At the end of the Last Ice Age and at the beginning of the Holocene, European larch was relatively common in Central Europe. In most critical period of the Middle Holocene, it demonstrably retreated to exposed rocky habitats, but did not become completely extinct. In the form of relict populations, it survived locally until the modern era, when non-native larch types were artificially introduced into managed forest stands. Our findings call for a radical rethinking of the approach to larch by both foresters and nature conservation authorities. In the future, it is primarily necessary to search for and protect the remnants of its original gene pool.
European larch (Larix decidua Mill.) is an important tree species commonly used in managed forests of central Europe. However, its genetic structure, post-glacial range dynamics, and consequent nativity status are poorly understood. Here we provide the first study focusing on the genetic structure of in situ larch populations in the north-eastern Czech Republic using molecular genetics. Most of the studied area is traditionally considered to be a part of the autochthonous native range of the Jeseníky lineage of larch and some of the included populations are protected as belonging to this type. We confirm a common presence of the Jeseníky type and its close evolutionary relation with larch populations from both the Carpathians and Poland. However, we also found important levels of admixture of genetic material from the Alps in all of the analyzed populations. The highest amount of Alpine admixture was found in the populations of intensively managed forests, the proportion of non-native material in the protected areas was relatively lower but still significant. Our results show the importance of populationgenetic research for practical conservation of local populations and lineages even for common species.
The paper contains the evaluation of 9 European black pine provenances on the research trial No. 41 – Roblín (Czech Republic) at the age of 51 years. The tested provenances originated from Bosnia and Herzegovina, Serbia, Croatia, Bulgaria, Greece, France and Slovakia. The evaluation is based on an assessment of following factors: total height, dbh, stem form, health state, defoliation, diameter of main branches, stem damage, crown density, twisted growth, stem nature pruning, cross-section, and taper. Obtained data sets were statistically analysed. The best growth both in quantitative and qualitative traits was recognised for the provenance 9 – Les Barres, Oise (France), which was excellent in height growth, health status and stem shape, and also for the Serbian provenance 2 – Crni Vrh, Titovo Užice, which excelled not only in growth rate, stem shape and health status, but also in diameter of main branches. Less suitable provenance seem to be 3 – Prušačka Rjeka, Banja Luka from Bosnia and Herzegovina (worse growth, stem nature pruning, cross-section and taper) and Croatian 5 – Južna Kandija, Osijek (worse growth, stem shape, and health). The most important for explaining the differences between provenances were the diameter at the breast height, crown density and defoliation.
This study examines the variability of Scots pine provenances in four Czech research plots from the same series of experiments conducted in 1972. 121 provenances from the Czech Republic and Slovakia were evaluated in terms of growth and morphological characteristics. The quantitative traits measured for each tree were its tree height and DBH. The stem volume was also calculated according to the volume equation (Petráš, Pajtík 1991). Among the qualitative traits, stem form, health status, branch thickness in the crown, stem damage, stem twist, stem clearing, stem cross-section, and taper were evaluated. The large variance in growth characteristics of provenances does not specify the suitability of a particular reproductive material for a given area. Slovak provenances even from a relatively small land unit (Šaštín area) planted in the same research plot have different quantitative and qualitative traits. However, the evaluation of the test plots of Scots pine in Bohemia showed impaired growth performance of most of the Slovak provenances. Therefore, it is necessary to test more provenances from a larger area of the Scots pine range to obtain the possibility of using a wider range of reproductive material of the tree in our conditions. In the long term, further provenance trials with new reproductive material are needed to assess suitability under changing natural conditions.
The paper deals with growth and prosperity of 25 provenances of grand fir from Washington, Oregon, Idaho and Montana (USA), and from Vancouver (British Columbia, Canada) tested in the international provenance research trial No. 212 – Drahenice (Czech Republic; N49° 31‘ 10‘‘, E13° 57‘ 20‘‘, 570 m a.s.l., acidic beech site). Biometric measurements took place at the age of 41 years; qualitative features were visually assessed according to the IUFRO methodology. The best overall assessment of both production and quality shows provenance 12041 – Oyster Bay (BC). Other provenances with high growing stocks, i.e. 12005 – Bear Mountain (WA), 12038 – Clearwater (ID), 12043 – Sproat Lake (BC) and 12044 – Kaye Road (BC) showed some qualitative shortcomings (lower vitality, worse stem shape or thicker branches). The worst both in production and quality were provenances 12020 – Crescent Creek (OR) and 12011 – Clear Lake (WA). The results confirm the suitability of the current setting of the rules for the transfer of reproductive material from North America to the Czech Republic.
In the middle of the twentieth century, a downward trend in grouse numbers in Central Europe became evident. Along with the decrease in the number of birds, the fragmentation and subsequent shrinking of the range of forest fowls such as the capercaillie (Tetrao urogallus), the black grouse (Lyrurus tetrix) and the hazel grouse (Bonasa bonasia) began to manifest itself. This trend continues until now and the occurrence of these species in the Czech Republic and Central Europe in general is threatened. One of the segments of saving the species in situ is the support of the population by artificially reared or translocated individuals. First of all, however, it is necessary to solve the problem of the biological quality of the individuals used, which largely depends on the state of their digestive tract, the quality of the food and the development of behavioral patterns necessary for the intake of natural food in the wild. Therefore, analyzes were made of the food supply of black grouse in the wild and potential feed components for black grouse in aviary breeding (Wendes method – dry matter, N substances, fats, ash, fibre, BNLV, expressed in %). Subsequently, on the basis of the analysis results, a recipe of a feed mixture of seeds, dried shoots, fruits of woody plants and dried insects was prepared, suitable for rearing and preparing grouse birds for adaptation to natural conditions. It presents a diversified food consisting of six types of cereals, two types of legumes, four types of oil seeds and another four types of seeds, fruits and dried shoots. The feed mixture of natural plant components was supplemented with an animal component – dried developmental stages of mealworm (Tenebrio molitor).
The climate change has an adverse impact on tree species and forest stands. Different tree species tolerance and changes in tree species composition are considered as one of the climate change consequences. The paper deals with assessment and comparison of tree species composition and basal area increment in mixed (spruce-fir-beech) stands, which were left to self-thinning during 45–55 years. The large-scale experimental basis of older thinning permanent research plots established, in the past, in mixed stands situated in the 5th and 6th altitudinal forest zone was used in the research. At the same time the detected changes were compared to long-range goal and tree species models specified for a given forest type. The results showed increased proportion of beech and sycamore maple contrary to decreased share of fir and spruce. At the same time, fir also recorded the highest percentage of loss by self-thinning. The comparison of the current tree species composition with the models showed slight differences in all areas in favour of spruce at the expense of beech. Similarly, the basal area increment of beech was the highest everywhere.