Popis článkuHodnocení semenného sadu třešně ptačí s využitím mikrosatelitových markerů.
[Evaluation of wild cherry seed orchard using microsatellite markers] 271 - 278.
|Název článku:||Hodnocení semenného sadu třešně ptačí s využitím mikrosatelitových markerů|
|Autor:||Pavlína Máchová, Helena Cvrčková, Eva Pokorná, Olga Trčková|
The Simple Sequence Repeats (SSR) method of DNA analyses was used to clonal identification of trees in a model wild cherry (Prunus avium L.) seed orchard. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from buds taken from 91 sampled trees of the seed orchard. Samples were screened using selected ten polymorphic nuclear microsatellite markers. Measuring of the size of amplification products was carried out using the genetic analyser Applied Biosystems 3500. The obtained data were analysed by statistical programs CERVUS, GenAlEx 6.501 and Micro-Checker. There were detected 65 different alleles at 10 loci in the 91 wild cherry individuals from seed orchard. By applying of the 10 suitable markers to the 18 clones from model seed orchard we obtained multilocus genotypes (MLG). The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual genotypes identification. Declared clone affiliation was confirmed in 94% of sampled trees. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of wild cherry trees.