ZLV Online

Abstrakt

The Simple Sequence Repeats (SSR) method of DNA analyses were used to clonal identification in a model Norway spruce (Picea abies /L./ Karsten) seed orchard. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from needles taken from 100 sampled trees of seed orchard. Samples were screened using selected nine polymorphic nuclear microsatellite markers. Measuring the size of amplification products was carried out using the genetic analyzer Applied Biosystems 3500. The obtained data were analyzed using the statistical programs CERVUS (KALINOWSKI et al. 2007) and GenAlEx 6.501 (PEAKALL, SMOUSE 2006, 2012). There were detected 115 different alleles at 9 loci in the 100 Norway spruce individuals from seed orchard. The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual identification. Declared clonal affiliation was confirmed in 94% of sampled trees. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of Norway spruce trees.