ZLV Online

Abstrakt

The Simple Sequence Repeats (SSR) method of DNA analyses was used to clonal identification of trees in a model lime (Tillia cordata Mill.) seed orchard. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from young leaves taken from 377 sampled trees of the seed orchard. Samples were screened using selected eight polymorphic nuclear microsatellite markers. Measuring of the size of amplification products was carried out using the genetic analyser Applied Biosystems 3500. The obtained data were analysed by statistical programs CERVUS, GenAlEx 6.503. There were detected 79 different alleles at 8 loci in the 377 lime individuals from seed orchard. By applying of the 8 suitable markers to the 38 clones from model seed orchard we obtained multilocus genotypes (MLG). The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual genotypes identification. 93.6 % of the sampled trees could be assigned to the clones represented in the seed orchard. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of lime trees.