Popis článkuVyužití DNA markerů pro kontrolu deklarovaného původu reprodukčního materiálu smrku ztepilého
[Use of DNA analyzes for verifying the declared origin of Norway spruce reproductive material] 292-301
|Využití DNA markerů pro kontrolu deklarovaného původu reprodukčního materiálu smrku ztepilého
|Pavlína Máchová, Helena Cvrčková, Olga Trčková
The purpose of this study was to investigate the possibilities of using objective methods of DNA analysis to verify the declared origin of reproductive material of Norway spruce in terms of the Czech Republic. Monitoring of the identity of reproductive material was carried out during three years, i.e., from seed collection to transplanted plants production. Reference samples from the 32 sets of reproductive material were obtained, analyses of microsatellite markers were performed, and the genetic compositions of sets were compared after statistical processing. Altogether, DNA analyses were performed on 1920 samples of plant material from 8 selected sources of forest reproductive material (units of forest reproductive material). Seven optimally polymorphic markers with sufficient informative value were selected for the subsequent evaluation of the genetic structure of the monitored sets of Norway spruce reproductive material by Bayesian clustering. Using the performed Structure analysis, the obtained profiles of 8 monitored units of reproductive material (4 sample sets from one units) of different origin were distinguishable from each other. Thus, these methodological procedures could be used in the state control systems to certify the origin of forest reproductive material and increase consumer protection of forest owners and nursery production in the Czech Republic.